Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide :: GCSE Chemistry Coursework Investigation

Investigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of henry bleachSafety=======* Extreme alkaline and acids are used so essential wear gloves, goggles and apron.* Be very careful not to halt contact with either of the chemicals, as they will be irritable and some corrosive to the skin and eyes.* There must be no running and any other normal laboratory rules should be followed as usual.* Hydrogen peroxide is corrosive and so should not be touched.* A lab technician should immediately wash up up spillages.* Always be careful of the glass instruments as these could break.Background knowledge In my experiment I will be measuring the beatof oxygen given of in a gas syringe over a certain time purpose fromdifferent pH levels.Hydrogen hydrogen peroxide is a chemical compound, H2O2, a colourless, syrupyliquid that is a strong oxidizing agent and an in water solvent aweak acid. It is miscible with stale water and is soluble in alcoholand ether. Although pure hydrogen peroxide is fairly stable, itdecomposes into water and oxygen when heat above about 80C it alsodecomposes in the presence of numerous catalysts e.g. most metals,acids.The properties and factors that effect breakdown of Hydrogen Peroxideare grand in this investigation. The reaction of Hydrogen Peroxideforms water and oxygen, which is shown in the equation below.2H2O2 2H2O + O2On its own this reaction is very slow and normally a catalyst isneeded to fixedness up the reaction. A catalyst is used to urge on upreactions without it getting used up. So a biological catalyst such asan enzyme is used to speed up the process in the equation above. In myexperiment I am going to do I am using dried yeast, which contains asuitable enzyme catalase. So therefore I am using the catalase inthe yeast to speed up the reaction, the products world produced andthe overall results.To explain why enzymes speed up reactions it is based on the collisiontheory. The enzymes, which are immobilis ed in the yeast-based beads,will give many active sites for the substratum (Hydrogen Peroxide) tobind (lock) with. This will extend the collisions and thereforethe rate of reaction.Immobilised enzymes can be washed and re-used. The beads are allrelatively the same sizing so that there is less venture of error on theinvestigation. Immobilised enzymes are also more stable in extremes oftemperature and pH, which it will need for my experiment.A fan solution is one, which resists changes in pH when lesserInvestigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen Peroxide GCSE Chemistry Coursework InvestigationInvestigate the Effect of pH on Immobilised Yeast Cells on the Breakdown of Hydrogen PeroxideSafety=======* Extreme alkaline and acids are used so must wear gloves, goggles and apron.* Be very careful not to make contact with any of the chemicals, as they will be irritable and some corrosive to the skin and eyes.* There must be no running and any other normal laboratory rules should be followed as usual.* Hydrogen peroxide is corrosive and so should not be touched.* A lab technician should immediately wash up spillages.* Always be careful of the glass instruments as these could break.Background knowledge In my experiment I will be measuring the amountof oxygen given of in a gas syringe over a certain time period fromdifferent pH levels.Hydrogen Peroxide is a chemical compound, H2O2, a colourless, syrupyliquid that is a strong oxidizing agent and an in water solution aweak acid. It is miscible with cold water and is soluble in alcoholand ether. Although pure hydrogen peroxide is fairly stable, itdecomposes into water and oxygen when heated above about 80C it alsodecomposes in the presence of numerous catalysts e.g. most metals,acids.The properties and factors that effect breakdown of Hydrogen Peroxideare important in this investigation. The reaction of Hydrogen Peroxideforms water and oxygen, which is shown in the equation b elow.2H2O2 2H2O + O2On its own this reaction is very slow and normally a catalyst isneeded to speed up the reaction. A catalyst is used to speed upreactions without it getting used up. So a biological catalyst such asan enzyme is used to speed up the process in the equation above. In myexperiment I am going to do I am using dried yeast, which contains asuitable enzyme catalase. So therefore I am using the catalase inthe yeast to speed up the reaction, the products being produced andthe overall results.To explain why enzymes speed up reactions it is based on the collisiontheory. The enzymes, which are immobilised in the yeast-based beads,will give many active sites for the substrate (Hydrogen Peroxide) tobind (lock) with. This will increase the collisions and thereforethe rate of reaction.Immobilised enzymes can be washed and re-used. The beads are allrelatively the same size so that there is less chance of error on theinvestigation. Immobilised enzymes are also more stable in extrem es oftemperature and pH, which it will need for my experiment.A buffer solution is one, which resists changes in pH when small